Slow off-rate modified aptamers
WebbSynthesis of 3′-inverted dT modified aptamers needs modified CPG with the 5′-hydroxyl of the first nucleoside attached, followed by chain elongation in standard 3′→5′ fashion. 3.1.2. 3′-Biotin Conjugates In some ways, 3′-biotin ( Figure 5) could resist the activity of 3′-exonuclease, which was similar to 3′-inverted dT modification. Webb29 okt. 2024 · For this purpose, we have generated four next-generation modified aptamers called SOMAmer ( S low O ff-rate M odified A pta mer) reagents that have different sequences and compositions,...
Slow off-rate modified aptamers
Did you know?
WebbWe sought to determine whether slow off-rate modified aptamer (SOMAmer) reagents with subnanomolar affinity for Mycobacterium tuberculosis proteins (antigens 85A, 85B, 85C, … Webb11 nov. 2024 · The hybridization requires the following steps: 95 °C for 10 min, 60 °C for 60 s, then cool slowly to 25 °C for 60 s at a rate of 0.1 °C/s. ... The aptamer loading step was a procedure of biotin-modified aptamers immobilizing ... immobilization of the hybrid on streptavidin beads; (4) washing off unbound ssDNA library; (5 ...
Webb2 aug. 2024 · The SOMAmers (Slow Off-rate Modified Aptamers) not only display improved binding affinities and binding kinetics (in particular, slow off-rates) when compared to traditional aptamers, but also the inclusion of these modifications in their libraries significantly increased the selection “hit rate” . WebbConsiderably, there are several kinds of aptamer-modification strategies to augment binding affinity and target selectivity: 1) Modifications on bases slow off-rate modified aptamers (SOMAmers): 5-BzdU [5-(N-benzylcarboxyamide)-2-deoxyuridine]-modified aptamer and SOMAmers containing 5-(N-benzylcarboxamide)-2-deoxyuridine (Bn-dU) or …
Webb1 maj 2011 · Aptamers can circumvent these limitations, but their application has been hindered by nonspecific binding to cellular components, particularly in the nucleus. Here … Webb15 feb. 2011 · The decrease in peak current was observed which could be attributed to slow kinetics of the charge transfer, which is caused by the binding of 17β-estradiol to aptamer [40,49]. However with the series of 17β-estradiol dilutions tested it was found out that 0.1 nM 17β-estradiol was detectable with a lower concentration of 1 nM ssDNA …
Webb6 sep. 2016 · Slow off-rate modified aptamers (SOMAmers) are attractive protein recognition reagents due to their high binding affinities, stable chemical structures, …
Webb25 apr. 2024 · demonstrating the generation of many high-affinity ‘slow off-rate modified aptamers’ (SOMAmers) and shown the utility of these reagents for generating reproducible measurements of circulating proteins.9,10 Unfortunately, the sequences of these aptamers are not available to the cihr project grant scoringWebb3 sep. 2024 · Slow off-rate modified aptamer proteomics is an attractive analytical tool for rapid proteomic stratification of different malignant gliomas and identified cluster … cihr project grant summary of progressWebbHere we describe unique slow off-rate modified aptamers that facilitate rapid and selective binding to target proteins in tissue. Specifically, we have developed a fluorescent … dhl first mileWebb7 juli 2015 · Rapid Slow Off-Rate Modified Aptamer (SOMAmer)-Based Detection of C-Reactive Protein Using Isotachophoresis and an Ionic Spacer We present an on-chip … cihr project grant reviewer manualWebb6 mars 2024 · Such doubly modified aptamers are also more likely to be encoded in shorter sequences and occupy nonoverlapping epitopes more frequently than aptamers … cihr publication policydhl first officersWebb13 apr. 2024 · It develops slow off-rate modified aptamers (SOMAmers), which are modified nucleic acid-based protein binding reagents that are specific for their cognate protein; and offers proprietary SomaScan ... cihr project mandates