WebMETHODS: Bronchoalveolar lavage fluid was obtained from a house dust mite (HDM) induced murine asthma model exposed to DEP. GSH, … WebWe, therefore, decided to repeat these asthma experiments by doubling HDM amounts for sensitization and quadrupling them for challenges (termed ‘HDM high model’ Figure S1A). Under these severe conditions, HDM-treated hIL-4/13/4Rα KI mice exhibited a marked increase in lung resistance and elastance upon exposure to methacholine, compared ...
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WebNov 9, 2024 · In this HDM-induced asthma model, we treated the mice with Ber and its NPs, during the sensitization and challenge stage of this chronic model . As shown in Figure 3B, Ber and its NPs reduced total infiltrated cells numbers in BALF of HDM-induced asthmatic mice. Among them, PM@Ber-NPs were the least in reducing cell infiltration … WebNov 20, 2024 · The dysfunction of airway epithelial barrier is closely related to the pathogenesis of asthma. Secreted Hsp90α participates in inflammation and Hsp90 inhibitor protects endothelial dysfunction. In the current study, we aimed to explore the role of secreted Hsp90α in asthmatic airway epithelial barrier function. Male BALB/c mice were …
WebMay 9, 2024 · Even in the same HDM-induced asthma mouse model, it is possible to create an acute asthma model, a chronic asthma model, and a remodeling model depending on the amount of antigen exposure and th e ... WebIn this study, by using a murine model of HDM-induced chronic asthma in vivo and TGF-β1-induced airway epithelial cells in vitro, we sought to explore the roles of PGRN in asthmatic airway remodeling and autophagy, including its mechanism. Our findings demonstrated that PGRN was a protective regimen for asthmatic airway remodeling and …
WebMar 9, 2024 · The mouse model of house dust mite (HDM)-induced allergic airways disease mimics many of the features of human asthma symptoms, including airway hyperreactivity and airway inflammation, and is increasingly used to elucidate asthma/allergic airways pathology and to evaluate new therapeutic agents . However, …
WebDec 10, 2024 · To establish an animal model of asthma that more closely reflects the human disease, asthma phenotypes induced by allergen administration routes other than intraperitoneal or subcutaneous injection have been investigated [13,14,15], such as inhaled or intranasal delivery, which occurs in human asthma [16, 17]. However, there have …
WebTo study the allergen-specific immune response in mouse HDM induced asthma models of human asthma, these kits are ideal for assaying serum IgG, IgG1, IgG2b, IgE, and IgM antibodies against HDM extracts and Dermatophagoides pteronyssinus Peptidase 1 (Der p1), one of the main HDM allergens. Chondrex Inc.'s asthma model page has … hematoxilina de harris ficha tecnicaWebAug 3, 2024 · Figure 2 HDM induces NLRP3 inflammasome activation in alveolar macrophages.(A, B) Flow cytometry analysis of the GFP fluorescence intensity in the … hematoxylin and eosin h\u0026e 染色HDM extract (Greer Laboratories, Lenoir, NC) was re-suspended in normal saline and introduced by intranasal instillation, with the mice lightly anesthetized with 5% isoflurane, using a 20–200 µL pipette tip (Fig. 1). The acute HDM protocol used 100 µg HDM (D. pteronyssinus or D. farinae) in 50 µL saline on days 0–4 … See more The animal protocols were approved by the University Health Network Animal Care Committee and conducted in accordance with the guidelines of the Canadian Council on Animal Care (CCAC). Female BALB/c mice (8–10 … See more Mice were anesthetized with ketamine (50 mg/kg i.p., Wyeth Animal Health, Guelph, ON, Canada) and xylazine (10 mg/kg i.p., Bayern Inc., Toronto, … See more Serum HDM-specific IgE was assessed using the antigen-capture ELISA method22. Briefly, 96 well plates (NUNC MaxiSorp1 plates, … See more After completion of the pulmonary function testing, mice were euthanized with an overdose of ketamine/xylazine, and blood was collected via cardiac puncture21,22. In a subset of mice, … See more hematoxilina merckWebMar 13, 2024 · Even in the same HDM-induced asthma mouse model, it is possible to create an acute asthma model, a chronic asthma model, and a remodeling model depending on the amount of antigen exposure and … hematoxilina phWebFeb 7, 2015 · Here, a murine model for HDM-allergic asthma was used that mimics human features of asthmatic disease such as HDM-induced AHR, airway inflammation and pulmonary cytokine release [11,35,36]. Airway responsiveness upon methacholine exposure in HDM-allergic mice was significantly increased when compared to HDM-PBS control mice. hematoxylene stain is used for:WebApr 7, 2024 · Activated airway epithelial cells are the major source of CXCL10 in the lungs , which has been shown to be involved in both bronchial inflammation and airway hyper-responsiveness in a mouse model of asthma . CXCL10 is thought to be induced by Th1 cytokine IFN-γ in a variety of cells that promotes Th1-biased airway inflammation . land rover discovery 2 exhaustWebThe House Dust Mite (HDM)-induced asthma model in the mouse is used to assess the in vivo efficacy of anti-asthma drugs. This model features many similarities to human … hematoxylin and eosin h\u0026e staining 染色